Additional file 4: Figure S3. of Salinomycin inhibits metastatic colorectal cancer growth and interferes with Wnt/β-catenin signaling in CD133+ human colorectal cancer cells

Combined treatment with Salinomycin and 5-FU reduces migration and invasion of murine CRC cells and induces necrotic cell death. To visualize tumor cell migration 0.5 × 106 MC38 (A) and CT26 (B) cells were cultured in 6-well plates until confluence. A scratch was created in the middle of the monolayer and cells were treated with Salinomycin (1 μM, 2 μM, 5 μM and 10 μM) and 1 μM 5-FU. Cell migration was assessed by phase-contrast microscopy and images were captured at the beginning of treatment and after 24 and 48 h at a magnification of 100. The open wound area after 48 h in cultured MC38 (C) and CT26 cells (D) was calculated and displayed as a summary of 3 independent experiments as mean ± SD; ** p

盐霉素（Salinomycin）与5-氟尿嘧啶（5-FU）联合治疗可降低小鼠结直肠癌（CRC）细胞的迁移与侵袭能力，并诱导细胞坏死。为可视化肿瘤细胞迁移过程，将0.5×10⁶个MC38（A）及CT26（B）细胞接种于6孔板中，培养至细胞汇合。随后在单层细胞中央制造划痕，并用盐霉素（1 μM、2 μM、5 μM及10 μM）联合1 μM 5-FU处理细胞。采用相差显微镜评估细胞迁移情况，分别于给药初始时刻、给药24小时及48小时时以100倍放大倍率拍摄图像。计算给药48小时后MC38（C）与CT26（D）细胞的开放划痕面积，并以3次独立实验的汇总数据呈现，结果以平均值±标准差（mean±SD）表示；**p