MOESM1 of Comparative transcriptomic and metabolic analysis of wild and domesticated wheat genotypes reveals differences in chemical and physical defense responses against aphids

Additional file 1: Table S1. Mapping sequence reads to the Chinese Spring reference genome. Table S2. Total RNA-seq values after rlog normalization. Annotations to the D subgenome or an unidentified subgenome (U) were eliminated. Table S3. Distribution of wheat genes into the eight clusters. Table S4. Gene annotation including the International Wheat Genome Sequencing Consortium database (IWGSC) and Phytozome gene ID. Table S5. Biological processes from the Singular Enrichment Analysis with agriGO v2 for significantly differentially expressed genes between each pair of genotypes. The data was divided into the eight k-means clusters. Only statistically significant GO terms are shown (FDR < 0.05). Queryitem: the number of genes containing the GO annotation; Querytotal: the total number of genes with GO annotations; bg item: the number of genes in wheat with this GO annotation; and bg total: the total number of genes in wheat with GO annotations. Table S6. Metabolites identified in leaves of 11-day-old wheat seedlings analyzed by GC-MS. The metabolites were normalized to the internal standard and presented as the relative abundance of the ion counts. Table S7. Weights of wheat leaf tissue used for water content calculation. The fresh, turgid and dry weights are measured in mg. Table S8. A full list of the Bx genes in bread wheat. The data include genes from Subgenome A, B, D, and U (not classified). Table S9. A full list of the trichome formation and regulation genes in bread wheat. The data include genes from Subgenome A, B, D, and U (not classified). Table S10. Benzoxazinoid annotation and fragment patterns detected and identified in wheat leaves by UPLC-QToF-MS analysis. Table S11. Levels of DIMBOA, DIM2BOA-Glc, and HDMBOA-Glc metabolites detected by HPLC-UV. Calibration curves were calculated by running authentic standards and crude extracts in different concentrations ranging from 0.5–50 μg/ml. The peak area of each compound was measured using Chromeleon software, and the final concentration was normalized to mg per gram fresh weight.