Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: AAEC191A/pACYCpBAD to polystyrene in a dynamic system

The attachment of bacteria begins the process of surface colonization, called biofilm development, characterized by a number of physicochemical and molecular interactions. Adherence to inert surfaces typically involves nonspecific interactions, whereas adherence to biological surfaces is associated with specific ligand-receptor interactions. In this study, the adherence ability of four genetically modified Escherichia coli strains—BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra, AAEC191A/pCC90, and AAEC191A/pACYCpBAD—was examined on both glass and polystyrene surfaces. The aim was to investigate the effect of the presence of Dr fimbriae on the adherence of bacteria to various carriers. The experiments were performed under various flow conditions.
The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

细菌附着是表面定殖过程的起始步骤，该过程被称为生物被膜（biofilm）形成，其特征为一系列物理化学与分子层面的相互作用。对惰性表面的黏附通常涉及非特异性相互作用，而对生物表面的黏附则与特异性配体-受体相互作用相关。本研究针对四种基因工程改造的大肠埃希氏菌（Escherichia coli）菌株——BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra、AAEC191A/pCC90以及AAEC191A/pACYCpBAD——在玻璃与聚苯乙烯两种表面的黏附能力展开了检测，旨在探究Dr菌毛（Dr fimbriae）的存在对细菌黏附各类载体的影响，实验在多种流动条件下开展。
本数据集包含原始视频数据，可通过奥林巴斯（Olympus）的CellSense软件进行播放。这些视频包含了表征图像记录过程的全分层数据。