BSA seq of waxless flowering Chinese cabbage and Chinese cabbage

To clarify the mechanism of the wax deficiency, the wax-less flowering Chinese cabbage doubled-haploid (DH) line CX001 and Chinese cabbage DH line FT, obtained from isolated microspore culture, were used in the experiments. Genetic analysis showed that the wax-less phenotype of CX001 was controlled by a recessive nuclear gene, named wlm1 (wax-less mutation 1), which was fine-mapped on chromosome A09 by bulked segregant analysis sequencing (BSA-seq) of B.rapa genome V3.0.