Post-eclosion temperature effects on insect cuticular hydrocarbon profiles

Body Mass Data: Body weight data was collected in the groups of five flies. The wet weight and dry weight data are given in miligrams. To obtain dry weight flies were dried at 50 degree centrigrade overnight. The wet mass, dry mass, and water content values were used to generate residuals for ANOVA analysis of desiccation tolerance. Cuticular Hydrocarbon Data: Hydrocarbons were extracted in hexane before running through gas chromatography column. Data file contains raw data (retention time). Peak areas of the GC chromatograms corresponding to individual CHCs were converted into relative proportions of the sum of CHC peak areas. The relative data was arcsine transformed and the overall quantitative variability among samples and treatments visualized using principal component analysis (PCA) performed in Statistica 8.0. Desiccation Assay: Flies in the groups of five were used for desiccation assay. To measure desiccation tolerance desiccating environemnt was created in the tube (using Silica gel). Data contains hourly dead flies number. We used LT100 data. We ran a mixed model ANOVA where population cages were nested within geographic source population. Respirometry Data (Water Loss Rate): Water-loss rate (WLR) was measured using flow-through respirometry. Flies in the groups were placed in a glass chamber where dry air was allowed to get in and outlet was connected with a humidity sensor. Water-loss rate was calculated from water vapor released by flies into the air stream. We ran a mixed model ANOVA where lines were nested within geographic source population.

体重数据集：以5只果蝇为一组收集体重数据。湿重与干重数据以毫克为单位。干重的获取方式为将果蝇置于50摄氏度环境中烘干过夜。基于湿重、干重与含水量生成残差，用于脱水耐受性的方差分析（ANOVA）。
表皮碳氢化合物数据集：采用正己烷萃取碳氢化合物后，通过气相色谱柱进行检测。数据文件包含原始检测数据（保留时间）。将气相色谱图中各表皮碳氢化合物(CHCs)的峰面积转换为占总峰面积的相对比例。对相对比例数据进行反正弦转换，使用Statistica 8.0软件完成主成分分析（PCA），以可视化样本与处理组间的整体定量变异程度。
脱水耐受性测定实验：以5只果蝇为一组开展脱水耐受性测定实验。通过硅胶在试管中营造干燥环境以检测果蝇脱水耐受性。数据集包含每小时的死亡果蝇数量，我们采用了LT100数据。使用混合效应方差分析模型进行统计检验，其中种群笼嵌套于地理来源种群中。
呼吸测定数据集（失水速率）：采用流通式呼吸测定法测量失水速率(WLR)。将果蝇置于玻璃测试舱中，通入干燥空气，出气口连接湿度传感器。通过果蝇释放到气流中的水蒸气量计算失水速率。使用混合效应方差分析模型进行统计检验，其中品系嵌套于地理来源种群中。