Pax3 plays a role in Neuro-2a cells proliferation and neurite outgrowth

Our fingdings suggested that Pax3 not only inhibited the cell viability and proliferation, but also affected the cell cycle and the neurite outgrowth of Neuro-2a cells.RNA sequencing analysis showed upregulated genes in Pax3 overexpressed group were involved in cell cycle machinery, which may reveal the potential mechanism of proliferation. Overall design: We use Pax3 overexpression adenoviral to infect Neuro-2a cells. to detect the function of proliferation,cell cycle and neurite outgrowth.We use Transcriptome sequencing to detect the expression level of mRNA in infected Neuro-2a cells.