Additional file 2: of Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves

Table S1. Differentially accumulated transcripts (DATs) upon MRCV infection at 12 and 21 dpi. For each gene, the normalized mean count for mock-inoculated and MRCV-infected samples, the fold change and the FDR-adjusted P-value (padj) are indicated. The annotations from Ensembl plants Release 37 or inferred in this work are included. Table S2. DATs classified as wheat Transcription factors according to reference [27]. Table S3. DATs classified as Carbohydrate-related according to their functional annotation. Table S4. Putative wheat invertases and fructosyltransferases, Tre6P synthases (TPS) and Sugars Will Eventually be Exported Transporters (SWEET) used to build the phylogenetic trees shown on Additional file 1: Figures S4-S6. Sequences encoded by DATs at 21 dpi are highlighted in red (increased upon the infection) and blue (decreased). A/N Inv, Vac Inv and CW Inv stand for acid and/or neutral, vacuolar and cell wall invertases respectively. Fructosyltransferases included sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferases (1-FFT) and sucrose:fructan 6-fructosyltransferases (6-SFT). Table S5. DATs classified as Amino acid-related according to their functional annotation. Amino acid transporters were extracted from reference [45]. Table S6. DATs classified as Hormone-related according to their functional annotation. Table S7. Primer sequences used for qRT-PCR validations. (XLSX 717 kb)

表S1. MRCV感染后12和21 dpi（接种后天数，days post inoculation, dpi）的差异积累转录本（Differentially accumulated transcripts, DATs）。针对每个基因，本表格列出了Mock接种组与MRCV感染组样本的标准化平均计数、折叠变化（fold change）以及经错误发现率（false discovery rate, FDR）校正的P值（padj）。同时收录了来自Ensembl Plants数据库第37版（Ensembl plants Release 37）的功能注释，或本研究中推断得到的注释信息。
表S2. 依据参考文献[27]归类为小麦转录因子（Transcription factors）的差异积累转录本。
表S3. 依据功能注释归类为碳水化合物相关（Carbohydrate-related）的差异积累转录本。
表S4. 用于构建附加文件1中图S4~S6所示系统发育树的推定小麦蔗糖转化酶、果糖基转移酶、海藻糖-6-磷酸合酶（Tre6P synthases, TPS）以及糖最终输出转运蛋白（Sugars Will Eventually be Exported Transporters, SWEET）。其中，21 dpi时差异积累转录本所编码的序列分别以红色（感染后表达上调）和蓝色（感染后表达下调）标注。A/N Inv、Vac Inv与CW Inv分别指代酸性/中性转化酶、液泡转化酶以及细胞壁转化酶。本次收录的果糖基转移酶包含蔗糖:蔗糖1-果糖基转移酶（sucrose:sucrose 1-fructosyltransferase, 1-SST）、果聚糖:果聚糖1-果糖基转移酶（fructan:fructan 1-fructosyltransferases, 1-FFT）以及蔗糖:果聚糖6-果糖基转移酶（sucrose:fructan 6-fructosyltransferases, 6-SFT）。
表S5. 依据功能注释归类为氨基酸相关（Amino acid-related）的差异积累转录本。其中氨基酸转运蛋白提取自参考文献[45]。
表S6. 依据功能注释归类为激素相关（Hormone-related）的差异积累转录本。
表S7. 用于qRT-PCR验证的引物序列。（附件格式：XLSX，文件大小：717 KB）