pat-2 functions in the muscle-mediated clearance of apoptotic MSpppaaa cells.
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aPercentage of embryos with the MSpppaaa cell corpse at the indicated developmental stage, with the number of embryos scored in parentheses.bPercentage of MSpppaaa cell corpses labeled with the MYRI::mRFP or PAT-2Δcyto::GFPcircle at the indicated developmental stage, with the number of embryos scored in parentheses.cEmbryos at the stage when pharyngeal grinder formation had just finished were scored.dHomozygous progeny of unc-79(e1068) pat-2(st567); Ex[Ppat-2pat-2::gfp] mothers.eHomozygous progeny of the heterozygous cdc-42(gk388)/mIn1[mIs14 dpy-10(e128)] mothers. The transgenic worms were generated as described in Materials and Methods.
a:在指定发育阶段下,携带MSpppaaa凋亡细胞的胚胎所占百分比,括号内标注了本次统计的胚胎数。 b:在指定发育阶段中,经MYRI::mRFP或PAT-2Δcyto::GFPcircle标记的MSpppaaa凋亡细胞所占百分比,括号内标注了本次统计的胚胎数。 c:统计对象为咽部磨器(pharyngeal grinder)刚完成形成阶段的胚胎。 d:实验样本为unc-79(e1068) pat-2(st567) 品系且携带附加型转基因阵列(extrachromosomal array, Ex)[Ppat-2pat-2::gfp]的母本所产生的纯合子代。 e:实验样本为杂合子cdc-42(gk388)/mIn1[mIs14 dpy-10(e128)]母本所产生的纯合子代。转基因线虫的构建方法详见材料与方法(Materials and Methods)部分。



