Microglia aging advances through intermediate states that drive inflammatory activation and cognitive decline

TGFB-signaling is important for the establishment of microglia homeostasis; however, it's role in aging has remained vague. We found that Tgfb1 and TGFB signaling is altered during hippocampal microglia aging. To investigate the effects that microglia-derived Tgfb1 has on microglia aging, we performed scRNA-Seq on Cd11b+ cells from the hippocampus of mature mice where Tgfb1 had been ablated from Cx3cr1 cells post-maturity. scRNA-Seq of Cx3cr1-CreERT Tgfb1 cKO Cd11b+ cells from mature mouse hippocampi