Minimum D-amino acid concentration required to prevent* germination by its equivalent L-amino acid.

Tests were conducted in 20 mM Tris buffer, pH 7.4, L-amino acid (1–10 mM), D-amino acid (10–100 mM) + L-lactate (50 mM) and NaHCO3 (50 mM) at 30°C. NT = D-phenylalanine was not inhibitory at 20 mM and could not be tested at a higher concentration due to low solubility.*Defined as a 600, equating to Minimum D-amino acid concentration required to prevent* germination by its equivalent L-amino acid.实验于30℃下在20 mM Tris缓冲液（Tris buffer，pH 7.4）中开展，反应体系包含L-氨基酸（1~10 mM）、D-氨基酸（10~100 mM）、L-乳酸盐（L-lactate，50 mM）及碳酸氢钠（NaHCO3，50 mM）。 NT指D-苯丙氨酸（D-phenylalanine）在20 mM浓度下未表现出抑制活性，且因溶解度较低，无法在更高浓度下进行测试。 *本指标以A600（600 nm处吸光度）为定义基准，即阻断其对应L-氨基酸诱导的萌发过程所需的最低D-氨基酸浓度。