Resveratrol-Induced Changes of the Human Adipocyte Secretion Profile

Enlarged white adipose tissue (WAT) is a feature of obesity
and leads to changes in its paracrine and endocrine function. Dysfunction
of WAT cells is associated with obesity-associated disorders like
type 2 diabetes and cardiovascular diseases. Resveratrol (RSV), a
natural polyphenolic compound, mimics beneficial effects of calorie
restriction. As such, RSV seems a promising therapeutic target for
obesity-associated disorders. The effect of RSV on the human adipokine
profile is still elusive. Therefore, a proteomic study together with
bioinformatical analysis was performed to investigate the effect of
RSV on the secretion profile of mature human SGBS adipocytes. RSV
incubation resulted in elevated basal glycerol release and reduced
intracellular TG content. This increased intracellular lipolysis was
accompanied by profound changes in the adipocyte secretion profile.
Extracellular matrix proteins were down-regulated while processing
proteins were mostly up-regulated after RSV treatment. Interestingly,
RSV induced secretion of proteins protective against cellular stress
and proteins involved in the regulation of apoptosis. Furthermore,
we found a RSV-induced up-regulation of adiponectin and ApoE accompanied
by a down-regulation of PAI-1 and PEDF secretion which may improve
anti-inflammatory processes and increased insulin sensitivity. These
effects may contribute to alleviate obesity-induced metabolic complications.
In addition, two novel RSV-regulated adipocyte-secreted proteins were
identified.