MS-based proteomic characterization of Toxoplasma gondii BCC7 interactome

Toxoplasma gondii is an obligate intracellular protozoan parasite of a wide range of homeotherm animals worldwide. T. gondii belongs to the ancient phylum of Apicomplexa and shares with the other companion groups from the Alveolata infrakingdom, a featuring trilamellar pellicle outlining the cell while a permanent apico-basal polarity also characterizes the T. gondii zoites. In addition to the plasma membrane bilayer, the pellicle also encompasses the underneath inner membrane complex (IMC) itself derived from flattened and fused vesicles identified as alveoli and made of a IMC and IMC-like protein’s repertoire, yet to complete. We identified a 500 kDa protein encoded by TGGT1_311230, the basal complex component BCC7, exhibiting a posterior localization. To gain insight about the role of BCC7 in T. gondii, we characterized its interactome.