SGA output for analysis sets 11-20.
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Query strains that express one effector were mated to an array of ~330 effectors in groups of ~10 queries at a time ("Analysis Set"). The arrays were then imaged using a high-resolution camera and the spot sizes were quantified using SGAtools (http://sgatools.ccbr.utoronto.ca/). Outlier spot sizes flagged by the Jackknife filter (JK) in SGAtools were removed and the average and standard deviation of the remaining values were calculated and normalized to the average empty vector control. This .zip archive includes spreadsheets that encompass the raw SGAtools data output from the paper for analysis sets 11-20.
将表达单种效应蛋白(effector)的查询菌株,每次以约10株为一组与包含约330种效应蛋白的阵列进行杂交,该组被称为‘分析集(Analysis Set)’。随后使用高分辨率相机对该阵列进行成像,并通过SGAtools(http://sgatools.ccbr.utoronto.ca/)对菌落斑点的大小进行定量分析。剔除SGAtools中通过刀切法过滤器(Jackknife filter,JK)标记的异常斑点大小数据,计算剩余数据的平均值与标准差,并以空载体对照(empty vector control)的平均值为基准进行归一化处理。本压缩包包含本研究中分析集11至20的原始SGAtools输出数据表格。
创建时间:
2016-12-13



