Single-cell RNA-seq profiles of lung adenocarcinoma and gastric cancer

single-cell RNA sequencing (scRNA-seq) profiles from eight patients with lung adenocarcinoma (LUAD) and six patients with gastric cancer (GC) were performed in this study. By integrating other scRNA-seq data and clinical information, we identified activated adaptive immune responses in older patients, reflected by enriched dysfunctional T cells marked by CXCL13, LAYN and immune checkpoint molecules, and tumor-associated macrophages (TAMs) marked by SPP1, CX3CR1 and CCL13. Our study shows increased efficacy of immune checkpoint blockade therapy in older patients, addressing the prominent role of age when considering immunotherapy.