Genome-wide analysis of galectin-4 mediated gene signature in PCa

Prostate cancer (PCa) causes significant mortality among men worldwide. Galectin-4 plays an important roles in regulatiog cancer metastasis and high level of galectin-4 correlated with poor survival in subpopulation of PCa patients. To further understand the galectin-4-mediated signature and develop effective treatment, we froced expressed galectin-4 in 22Rv1 and LNCaP cells, and downregulated galectin-4 in 22Rv1-M4 cells. Next, cDNA microarray were applyed to study galectin-4-mediated gene expression and cancer progression. To further study galectin-4 effect, galectin-4 gene was forced expressed in 22Rv1 and LNCaP, and downregulated in 22Rv1-M4. Total RNA expression were analyzed in cDNA microarray.