Effect of 3,5-diiodothyronine on rat-liver mitochondrial respiratory rates from lipid substrates.

Palmitoyl-CoA+carnitine or L-palmitoylcarnitine were used as respiratory substrates. Mitochondrial respiratory activity in state 3, state 4 and with FCCP is expressed as natoms oxygen/min/mg mitochondrial proteins. Respiratory control index (RCI) represents the ratio between state 3 and state 4 respiration rate. Data are means ± SD of 5 experiments performed with duplicate samples.本实验以棕榈酰辅酶A+肉碱（Palmitoyl-CoA+carnitine）或L-棕榈酰肉碱（L-palmitoylcarnitine）作为呼吸底物。线粒体呼吸活性在状态3（state 3）、状态4（state 4）及添加羰基氰化物-对-三氟甲氧基苯肼（FCCP）的条件下，均以氧原子数/分钟/毫克线粒体蛋白为单位进行表征。呼吸控制指数（Respiratory Control Index, RCI）为状态3与状态4呼吸速率的比值。实验数据为5次独立实验的均值±标准差，每次实验均采用双样本平行测定。