Differential gene expression analysis between adherent U87MG cells and U87MG cell-derived glioma stem-like cells (P4E8 cells).. Differential gene expression analysis between adherent U87MG cells and U87MG cell-derived glioma stem-like cells (P4E8 cells).

We established tumorspheres from human glioblastoma U87MG cells by single cell-derived tumorsphere formation. Among these tumorspheres, P4E8 clone showed cancer stem cell like properties such as self-renewal capacity, expression of cancer stem cell markers, resistance to anti-cancer agents and in vivo tumorigenicity. To find novel therapeutic target molecules, we performed differential gene expression analysis between U87MG and P4E8 cells. Overall design: RNA samples harvested from three separate dishes of U87MG cells and P4E8 cells were used for microarray analysis.