Data for: Triose phosphate utilization stress during photosynthesis addressed with dynamic assimilation measurements

Oscillations in CO2 assimilation rate and associated fluorescence parameters have been observed alongside the triose phosphate utilization (TPU) limitation of photosynthesis for nearly 50 years. However, the mechanics of these oscillations are poorly understood. Here we utilize the recently developed Dynamic Assimilation Techniques (DAT) for measuring the rate of CO2 assimilation to increase our understanding of what physiological condition is required to cause oscillations. We found that TPU limiting conditions alone were insufficient, and that plants must enter TPU limitation quickly to cause oscillations. We found that ramps of CO2 caused oscillations proportional in strength to the speed of the ramp, and that ramps induce oscillations with worse outcomes than oscillations induced by step change of CO2 concentration. An initial overshoot is caused due to a temporary excess of available phosphate. During the overshoot, the plant out-performs steady state TPU and ribulose 1,5-bisphosph..., This data was collected in laboratory in East Lansing MI using LI-COR LI-6800 instruments capable of dynamic assimilation measurements and modified to be able to take optical and fluorescent measurements. Data was processed using R scripts and collated into tables that are presented in this dataset., All data is presented in comma delimited tabular text.

近50年来，科研人员在光合作用受磷酸丙糖利用（TPU，triose phosphate utilization）限制的条件下，观测到了二氧化碳同化速率及其相关荧光参数的振荡现象。然而，此类振荡的作用机制仍未得到充分阐明。本研究采用新近开发的动态同化技术（DAT，Dynamic Assimilation Techniques）测定二氧化碳同化速率，旨在深入解析引发此类振荡所需的生理条件。研究结果显示，仅处于TPU限制条件下并不足以引发振荡，植物必须快速进入TPU限制状态，方可诱导振荡产生。此外，我们发现二氧化碳浓度斜坡变化引发的振荡强度与斜坡变化速率呈正相关，且相较于二氧化碳阶跃变化诱导的振荡，斜坡变化诱导的振荡会带来更不利的生理结果。初始超调现象源于可利用磷酸盐的暂时性过剩。在超调阶段，植物的光合表现会超越稳态TPU及核酮糖1,5-二磷酸（ribulose 1,5-bisphosph...）的相关水平。本数据集的数据采集于密歇根州东兰辛的实验室，使用具备动态同化测量功能、经改造可同步获取光学与荧光测量数据的LI-COR LI-6800仪器完成采集。数据通过R脚本进行处理，并整理为表格形式纳入本数据集。所有数据均以逗号分隔的表格文本格式呈现。