Effect of the Pta-AckA pathway and carbon sources availability on the activation of BfmR in the absence of BfmS.

In all panels, MPAO1, ΔbfmS, and ΔbfmSΔackA-pta harbor plasmid PAK1900, respectively. A) The relative expression of bfmR-lux in the wild-type MPAO1, the ΔbfmS strain, the ΔbfmSΔackA-pta strain, and its complementary strain (ΔbfmSΔackA-pta/p-ackA-pta) when bacteria were grown in M8-glutamate minimal medium supplemented with 0.2% glucose at 37°C for 24 h with shaking (250 rpm). Values are relative to MPAO1 (set to 1). B) Relative amount of C4-HSL measured by the pDO100 (pKD-rhlA) system. MPAO1 and its derivatives were grown in M8-glutamate minimal medium supplemented with 0.2% glucose at 37°C for 24 h with shaking (250 rpm). Supernatants were subsequently prepared and measured for the relative C4-HSL contents. Values represent means ± SEM. The assays were independently repeated at least three times with similar results obtained, and the graphs show a set of representative data.