Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune-suppressive activity of mesenchymal stromal cells

Figure 1. (A) Expression of Il1α mRNA in Sq-1979 cells treated with different serum concentrations. (B) Expression of Il1α mRNA in Sq-1979-1 cells under normoxia or hypoxia. Sq-1979-1 cells were sealed in tedlar bag with normal (20%) or hypoxic (1%) mixed air, seeded in the media containing 10% or 1% FBS for 36h.

Figure 2. Expression of IL-1α protein in Sq-1979-1 cells treated with 10% or 1% FBS under normoxic or hypoxic conditions. JPG file for Western blots.

Figure 3. (A) Release of IL-1α protein from Sq-1979-1 cells treated with different FBS concentrations. (B) Release of IL-1α protein from Sq-1979-1 cells under normoxia or hypoxia treated with 10% or 1% FBS. Sq-1979-1 cells were seeded in E-MEM supplemented with 10% or 1% FBS under normoxia or hypoxia for 36h.

Figure 4. (A) Effects of CMs prepared in lower serum conditions upon the promotion of immune suppressive activities of 10T1/2 cells. (B) Effects of CMs prepared under hypoxia upon the promotion of immune suppressive activities of 10T1/2 cells.

Figure 5. (A) Effect of IL-1α removal from CM prepared in low serum upon the promotion of immune suppressive activities of 10T1/2 cells. Relative IFN-γ-producing capability (%) of spleen cells was calculated as described in figure 4 legends. (B) Effect of IL-1α removal from CM prepared in hypoxia upon the promotion of immune suppressive activities of 10T1/2 cells.

These are datafiles of above titled manuscript.

图1. (A) 不同血清浓度处理的Sq-1979细胞中Il1α信使核糖核酸（Il1α mRNA）的表达水平。(B) 常氧（normoxia）或低氧（hypoxia）条件下Sq-1979-1细胞中Il1α mRNA的表达水平。将Sq-1979-1细胞封装于泰德拉袋（tedlar bag）中，充入正常（20%氧浓度）或低氧（1%氧浓度）混合空气，接种于含10%或1%胎牛血清（FBS）的培养基中培养36小时。

图2. 常氧或低氧条件下经10%或1% FBS处理的Sq-1979-1细胞中IL-1α蛋白的表达情况。附蛋白质免疫印迹（Western blots）的JPEG格式文件。

图3. (A) 不同FBS浓度处理的Sq-1979-1细胞释放的IL-1α蛋白水平。(B) 经10%或1% FBS处理的Sq-1979-1细胞在常氧或低氧条件下释放的IL-1α蛋白水平。将Sq-1979-1细胞接种于添加了10%或1% FBS的伊格尔最低限度必需培养基（E-MEM）中，于常氧或低氧条件下培养36小时。

图4. (A) 低血清条件下制备的条件培养基（CM）对10T1/2细胞免疫抑制活性的促进作用。(B) 低氧条件下制备的条件培养基（CM）对10T1/2细胞免疫抑制活性的促进作用。

图5. (A) 低血清条件下制备的条件培养基去除IL-1α后，对10T1/2细胞免疫抑制活性的促进作用变化。脾脏细胞产生干扰素γ（IFN-γ）的相对能力（%）按图4图例所述方法计算。(B) 低氧条件下制备的条件培养基去除IL-1α后，对10T1/2细胞免疫抑制活性的促进作用变化。

本数据集为上述标题稿件的配套数据文件。