Supplementary Information files for Rapid quantification of prion proteins using resistive pulse sensing

Supplementary Information files for Rapid quantification of prion proteins using resistive pulse sensing<br>Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB’s termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the<br>本数据集为《基于电阻脉冲传感（resistive pulse sensing，RPS）快速定量朊蛋白》的补充信息文件。 朊病毒病是一类由内源性细胞朊蛋白（PrPC）构象改变所引发的致死性传染性神经系统疾病。本研究开发了一种结合适配体与电阻脉冲传感（RPS）的快速检测方法，可从复杂样品基质中提取并定量内源性细胞朊蛋白。 研究人员将DNA适配体修饰于超顺磁珠（SPBs）表面。首先将此类磁珠（命名为P磁珠）用于从大体积样品中预富集分析物，随后将结合于P磁珠上的PrPC蛋白洗脱，再加入适配体修饰的传感微珠（S-beads）。S-beads通过纳米孔时的运动速度可反映其浓度。