In-vitro CCM tube formation

This set includes segmented images of the control cell (wild-type HUVEC) and cells with knockdown (KD) of the three Cerebral Cavernous Malformation (CCM) proteins, CCM1 (or KRIT1), CCM2, and CCM3 (or PDCD10), which disrupts the integrity of multicellular mesh. In addition, the control and KD cells were treated with an inhibitor of Rho-associated protein kinase (ROCK), which was shown to be over-activated in CCM KD cultures (Chernaya O, Zhurikhina A, Hladyshau S, Pilcher W, Young KM, Ortner J, Andra V, Sulchek TA, Tsygankov D. 2018. Biomechanics of Endothelial Tubule Formation Differentially Modulated by Cerebral Cavernous Malformation Proteins. iScience 9:347-358.8)

本数据集收录了对照细胞（野生型人脐静脉内皮细胞（wild-type HUVEC））与三种脑海绵状畸形（Cerebral Cavernous Malformation, CCM）蛋白敲低（knockdown, KD）细胞的分割图像，这三种CCM蛋白分别为CCM1（亦称KRIT1）、CCM2及CCM3（亦称PDCD10），该类蛋白的敲低会破坏多细胞网状结构的完整性。此外，对照细胞及CCM KD细胞均经Rho相关蛋白激酶（Rho-associated protein kinase, ROCK）抑制剂处理；既往研究已证实，ROCK在CCM KD培养体系中存在过度激活现象（Chernaya O, Zhurikhina A, Hladyshau S, Pilcher W, Young KM, Ortner J, Andra V, Sulchek TA, Tsygankov D. 2018. Biomechanics of Endothelial Tubule Formation Differentially Modulated by Cerebral Cavernous Malformation Proteins. iScience 9:347-358.8）