Table_1_Contrasting Nitrogen Fertilisation Rates Alter Mycorrhizal Contribution to Barley Nutrition in a Field Trial.xlsx

Controlled environment studies show that arbuscular mycorrhizal fungi (AMF) may contribute to plant nitrogen (N) uptake, but the role of these near-ubiquitous symbionts in crop plant N nutrition under natural field conditions remains largely unknown. In a field trial, we tested the effects of N fertilisation and barley (Hordeum vulgare L.) cultivar identity on the contribution of AMF to barley N uptake using 15N tracers added to rhizosphere soil compartments. AMF were shown capable of significantly increasing plant 15N acquisition from root exclusion zones, and this was influenced by nitrogen addition type, N fertiliser application rate and barley cultivar identity. Our data demonstrate a previously overlooked potential route of crop plant N uptake which may be influenced substantially and rapidly in response to shifting agricultural management practices.

在受控环境的研究中，共生根瘤菌（AMF）可能对植物氮素（N）的吸收有所贡献，然而，这些几乎无处不在的共生体在自然田间条件下对作物植物氮营养的作用仍鲜为人知。在田间试验中，我们通过向根际土壤室添加^15N示踪剂，检验了氮肥施用和 barley（Hordeum vulgare L.）品种对共生根瘤菌对 barley 氮素吸收贡献的影响。研究表明，共生根瘤菌能够显著增加植物从根际排除区域获取^15N的能力，这一现象受到氮添加类型、氮肥施用率和 barley 品种的影响。我们的数据揭示了作物植物氮素吸收的一个先前被忽视的潜在途径，这一途径可能对农业管理实践的转变产生显著而快速的影响。