CIL:34903

Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phalloidin, fixed with glutaraldehyde, treated with 33% methanol, and immunostained with p21 antibody followed by 10-nm gold-conjugated secondary antibody. Image corresponds to a single panel from Figure 4 in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 4 are available as CIL:34902-34921.肌动蛋白丝（actin filament）分支位点的Arp2/3复合物（Arp2/3 complex）定位。实验以爪蟾角膜细胞与成纤维细胞为材料，经CD处理（0.2 μM 处理30分钟，或0.5 μM 处理10分钟）后，在鬼笔环肽（phalloidin）存在下进行抽提，再以戊二醛（glutaraldehyde）固定，经33%甲醇处理后，使用p21抗体进行免疫染色，随后采用10 nm金偶联二抗进行标记。本图像为《Journal of Cell Biology》（J Cell Biol）1999年5月31日刊发的第145卷第5期第1009-1026页中图4的单幅子图。图4的全部子图均可通过CIL:34902-34921获取。