Identification of species in the angiosperm family Apiaceae using DNA barcodes

Apiaceae (Umbelliferae) is a large angiosperm family that includes many medicinally important species. The ability to identify these species and their adulterants is important, yet difficult to do so because of their subtle fruit morphological differences and often lack of diagnostic features in preserved specimens. Moreover, dried roots are often the official medical organs, making visual identification to species almost impossible. DNA barcoding has been proposed as a powerful taxonomic tool for species identification. The Consortium for the Barcode of Life (CBOL) Plant Working Group has recommended the combination of rbcL+matK as the core plant barcode. Recently, the China Plant BOL Group proposed that the nuclear ribosomal DNA internal transcribed spacer (ITS), as well as a subset of this marker (ITS2), be incorporated alongside rbcL+matK into the core barcode for seed plants, particularly angiosperms. In this study, we assess the effectiveness of these four markers plus psbA-trnH a...

伞形科（Apiaceae，Umbelliferae）是一类大型被子植物科，涵盖诸多具有重要药用价值的物种。准确识别这些物种及其混伪品虽意义重大，但因果实形态差异细微，且馆藏标本常缺乏鉴别特征，该任务极具难度。此外，该类群的法定药用部位多为干燥根，使得依靠视觉形态鉴定至物种级别几乎无法实现。DNA条形码（DNA barcoding）已被提议作为一种高效的物种鉴定分类学工具。生命条形码联盟（CBOL）植物工作组推荐将rbcL+matK组合作为植物核心条形码。近期，中国植物条形码研究组提出，应将核糖体DNA内转录间隔区（ITS）及其亚区域标记ITS2与rbcL+matK一同纳入种子植物（尤其是被子植物）的核心条形码体系。本研究对上述4个标记以及psbA-trnH的鉴定效能展开评估……