Gradients2-MGL1704-FluorometricChlorophyll_2020_04-01_v1.0

Cruise: Gradients 2, MGL1704 Project Name: Simons Foundation, Gradients 2 NPSG Description: Fluorometric Chlorophyll were collected from rosette nisken bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80°C until analysis. Post cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20°C freezer, covered to prevent the photodegradation of pigments. Upon extraction samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data were corrected for average glass fiber filter blank values and when available is reported as the average of duplicate samples (NaN indicates no duplicate sample).航次：Gradients 2，编号MGL1704；项目名称：西蒙斯基金会（Simons Foundation） Gradients 2 NPSG 说明：荧光测定叶绿素（Fluorometric Chlorophyll）样品采自罗塞特-尼斯肯采水瓶（rosette nisken bottles）的指定深度层，经玻璃纤维滤膜（glass fiber filter）以低真空条件过滤。随后将样品置于液氮中快速冷冻，装入Histoprep冻存管（histoprep capsule）后于-80℃保存直至分析。 航次结束后，将滤膜置于-20℃冰箱中用90%丙酮萃取48小时，全程避光以防止色素发生光降解（photodegradation）。萃取完成后对样品进行离心以沉降滤膜颗粒物，随后小心转移至玻璃比色皿（glass cuvette）中。随后使用Turner 10-AU型荧光光度计（Turner 10-AU Fluorometer）对样品进行检测。 所有数据均已通过玻璃纤维滤膜空白平均值校正；若存在平行样品，则以平行样平均值上报，NaN表示无平行样品。