Single cell RNASeq of whole mouse kidneys from Uromodulin/Tamm horsfall protein knock-out mice

To elucidate changes in cellular populations in the ischemic acute kidney injury, we performed single cell RNAseq on mouse kidneys following ischemic reperfusion injury and a sham surgery. Mice were subjected to bilateral clamping of the renal pedicule for 30 minutes. After 6-7 hours of reperfusion the kidneys were removed and processed by a MACs cell isolation protocol and dead cell removal (PMID:33448928) , sorted on a 10X Chromium platform version 3 and libraries generated. Sequencing was performed on an Illumina NovaSeq 6000. Overall design: Uromodulin/Tamm horsfall protein knock-out 129ev mice were subjected to a Sham surgery or bilateral renal clamping for 30 minutes.