Euphorbia esula Gene-space sequencing and assembly

Leafy spurge has been used as a model organism for studying dormancy in perennial herbaceous plants and other weediness traits. The goals of this project were to create a genomic resource for leafy spurge and to assemble the gene-space as best we could from our limited sequences. SAMN07691944 contains two different PE libraries from a collection of shoot tips from one cloned individual SAMN07691961 contains two different PE libraries from a collection of shoot tips from a (potentially) different cloned individual SAMN07693206 contains PE reads from a set of 12 different ChIP-seq libraries that showed very poor enrichment for the target sequence but which therefore contained a lot of genomic sequence data. These libraries were made from bud samples from an uncharacterized mix of individuals from a small population of plants that had been maintained clonally for many years. These samples were cleaned and processed and then used in two different assembly protocols- one in which the gene-space sequences were selected prior to assembly (PRJNA413193) and the other assembly was from the total set of cleaned fragments for whole genome assembly (PRJNA411945).乳浆大戟（Leafy spurge）已被用作研究多年生草本植物休眠及其他杂草性状的模式生物（model organism）。本项目的目标是为乳浆大戟构建基因组资源，并依托有限的测序数据尽可能组装出其基因空间（gene-space）。 SAMN07691944包含来自单个克隆个体的茎尖样本集合的两种不同双端（Paired-End, PE）测序文库；SAMN07691961包含来自另一（潜在）不同克隆个体的茎尖样本集合的两种不同双端测序文库。 SAMN07693206包含12个不同染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq）文库的双端读段，这些文库的靶序列富集效果极差，但因此蕴含了大量基因组序列数据。该批文库由来自一个经多年无性繁殖保种的小型植物种群的未鉴定混合个体的芽样本构建。 上述样本经净化与处理后，被用于两种不同的组装流程：其一在组装前先筛选基因空间序列（对应项目编号PRJNA413193），其二则使用全部净化后的片段开展全基因组组装（对应项目编号PRJNA411945）。