Additional file 1 of Biological evaluation of novel gemcitabine analog in patient-derived xenograft models of pancreatic cancer

Additional file 1: Supplementary Fig 1. A (SF 1A). HPLC spectrum for detecting the purity of 4NSG at 98.8% with a wavelength of 254 nm. B (SF 1B). Nuclear magnetic resonance spectrum for 4NSG. (a) 4NSG proton nuclear magnetic resonance (1H NMR) peaks at 10.88 ppm (-CO-NH) and 1.38–1.04 ppm (-CH2)15 representing the amide linkage and long-chain methylene group contributed by stearic acid respectively. (b) amide carbonyl carbon in 4NSG displays a characteristic C-13 NMR peak at 174.55 ppm. C (SF 1C). Mass spectrum for 4NSG at 530.59 m/z.

补充材料1：补充图1A（SF 1A）：以254 nm波长检测4NSG纯度的高效液相色谱（High Performance Liquid Chromatography, HPLC）谱图，其纯度达98.8%。
补充图1B（SF 1B）：4NSG的核磁共振谱图：
(a) 4NSG的质子核磁共振（proton nuclear magnetic resonance, ¹H NMR）谱峰：10.88 ppm处对应-CO-NH（酰胺键）特征峰，1.38~1.04 ppm处对应由硬脂酸贡献的(-CH₂)₁₅长链亚甲基基团特征峰。
(b) 4NSG中的酰胺羰基碳在174.55 ppm处呈现特征碳-13核磁共振（carbon-13 nuclear magnetic resonance, ¹³C NMR）峰。
补充图1C（SF 1C）：4NSG的质谱图，其特征质荷比为530.59 m/z。