ITGB4 in Human A375 Melanoma Constricted Migration -- Imaging and Flow Cytometry Datasets

This data accompanies the iScience publication: Playter et al. 2025 "Deciphering Pre-existing and Induced 3D Genome Architecture Changes involved in Constricted Melanoma Migration". Preprint available at BioRxiv (see link below) and accepted manuscript (see link below) contain all detailed methods for reproducing the data and figures made from the raw data included here. The ITGB4Data_README.docx file explains all the data and files included.
These data support the investigation of changes in genome architecture associated with constricted migration. Metastatic cancer cells traverse constricted spaces that exert forces on their nucleus and the genomic contents within. Cancerous tumors are highly heterogeneous and not all cells within them can achieve such a feat. In this study, we investigated what initial genome architecture characteristics favor the constricted migratory ability of cancer cells and which arise only after passage through multiple constrictions. We identified a cell surface protein (ITGB4) whose expression correlates with increased initial constricted migration ability in human melanoma A375 cells. Sorting out this subpopulation allowed us to identify cellular and nuclear features that pre-exist and favor migration, as well as alterations that only appear after cells have passed through constrictions. Data deposited here show the raw data for each cell sorting and flow cytometry experiment labeling ITGB4, images showing the phenotype and nuclear shape of melanoma cells that express or do not express ITGB4, and data tracking the migration efficiency of these cell populations. Our study reveals 3D genome structure contributions to both selection and induction mechanisms of cell fate change during cancer metastasis.

本数据集配套发表于学术期刊《iScience》的研究论文：Playter及其团队2025年发表的《解析与黑色素瘤受限迁移相关的预存在及诱导性三维基因组结构变化》。BioRxiv平台上的预印本（详见下方链接）以及已接收的正式手稿（详见下方链接）中包含了复现本数据集及基于本原始数据制作图表所需的全部详细实验方法。ITGB4Data_README.docx文件将对本数据集包含的所有数据与文件进行详细说明。
本数据集旨在支持与受限迁移相关的基因组结构变化研究。转移性癌细胞会穿过会对自身细胞核及内部基因组物质施加机械作用力的受限空间。癌组织具有高度异质性，并非所有肿瘤细胞都具备完成此类迁移行为的能力。本研究旨在探究：哪些初始基因组结构特征更有利于癌细胞的受限迁移能力，以及哪些特征仅在细胞多次穿过受限空间后才会出现。我们鉴定出一种细胞表面蛋白（ITGB4），其表达水平与人类黑色素瘤A375细胞的初始受限迁移能力增强呈正相关。通过分选该细胞亚群，我们得以识别出预先存在且有利于迁移的细胞及细胞核特征，以及仅在细胞穿过受限空间后才会出现的细胞改变。本数据集收录的原始数据包括：每一次针对ITGB4标记的细胞分选与流式细胞术实验数据、表达或不表达ITGB4的黑色素瘤细胞的表型及细胞核形态图像，以及追踪上述细胞群体迁移效率的相关数据。本研究揭示了三维基因组结构在癌症转移过程中，对细胞命运改变的选择与诱导两种机制均具有调控作用。