A Golden Gate Modular Cloning Toolbox for Plants

Plant
Synthetic Biology requires robust and efficient methods for
assembling multigene constructs. Golden Gate cloning provides a precision
module-based cloning technique for facile assembly of multiple genes
in one construct. We present here a versatile resource for plant biologists
comprising a set of cloning vectors and 96 standardized parts to enable
Golden Gate construction of multigene constructs for plant transformation.
Parts include promoters, untranslated sequences, reporters, antigenic
tags, localization signals, selectable markers, and terminators. The
comparative performance of parts in the model plant Nicotiana
benthamiana is discussed.