BRIC-21 Bacillus subtilis transcriptome profile data

The BRIC-21 mission was designed to identify the response of Bacillus subtilis to the human spaceflight environment. For this mission, samples were grown in rich-medium using the Biological Research in Canister Petri Dish Fixation Units (BRIC-PDFU) spaceflight hardware. B. subtilis spores were inoculated during spaceflight, grown at the ambient ISS temperature, and frozen in the onboard -80 C freezer prior to returning to Earth. RNA was extracted from samples grown onboard the International Space Station (ISS) and matching Ground Controls for transcriptome analysis.

BRIC-21任务旨在探究枯草芽孢杆菌（Bacillus subtilis）对载人航天环境的应答反应。本任务借助舱式生物研究培养皿固定单元（Biological Research in Canister Petri Dish Fixation Units，简称BRIC-PDFU）航天硬件，采用富集培养基完成样本培养。实验期间，于航天飞行过程中接种枯草芽孢杆菌芽孢，在国际空间站（International Space Station, ISS）的环境温度下进行培养，并在返回地球前，于舱内-80℃冷冻柜中完成冷冻保存。研究人员从国际空间站舱内培养的样本及匹配的地面对照组样本中提取RNA，用于转录组分析。