Temperature dependent growth and inorganic ion composition of haemolymph of juvenile Sepia officinalis, 2006

B Body wet weight and mantle length of juvenile Sepia officinalis were monitored over a peroid of five weeks. The animals had hatched in our aquarium system in Bremerhaven, Germany at 16°C and were descendants of individuals collected in the Oosterschelde estuary, Netherlands. Animals were kept in natural sea water at 10 or 17°C and fed small live shrimp (Palaemonetes varians) ad libitum daily. At the end of the experiment some animals kept at 17°C were sacrificed using ethanol. Haemolymph was withdrawn from the head vein using syringe and needle. Haemolymph samples were stored at -20°C until Na+, Cl-, K+, Mg2+, Ca2+ and SO42- concentrations were determined using ion chromatography. Mean body weight more that tripled at 17°C during the investigation period, while growth was impared by exposue to 10°C. Haemolymph ion concentrations were similar to those in sea water, except for sulphate. The concentration of this ion in the haemolymph was more that ten times lower than in sea water.

本研究对幼年期普通乌贼（Sepia officinalis）的体湿重与外套膜长度开展了为期五周的监测。实验个体于德国不来梅哈芬的本实验室水族系统内孵化，孵化水温控制为16℃，其亲本采集自荷兰奥斯特谢尔德河口。实验动物分别饲养于10℃或17℃的天然海水中，每日自由摄食活体变额长臂虾（Palaemonetes varians）作为饵料。实验结束时，对部分饲养于17℃条件下的个体采用乙醇实施安乐死，通过注射器与针头从头静脉采集血淋巴样本。血淋巴样本保存于-20℃环境中，后续采用离子色谱法测定其中Na+、Cl-、K+、Mg2+、Ca2+及SO42-的浓度。本研究周期内，17℃组的平均体重量增幅超过三倍，而10℃组的生长受到显著抑制。血淋巴中的离子浓度与天然海水基本一致，仅硫酸根离子除外——该离子在血淋巴中的浓度较海水中低十倍以上。