MT_eukDB

Eukaryote-derived PolyA-extracted metatranscriptome (‘MT-eukDB’), originating from 423,409,432 raw Illumina reads (~63.5 Gbps) recovered from the fungal community that colonized milled switchgrass during rumen-incubation. After quality filtering and assembly of the raw reads, we identified a total of 4,581,844 expressed genes for which 4,550,231 (99.31%) were predicted to encode proteins. The assembled metatranscriptome was filtered against the genome of the available cultured fungi (per 2017) before the MT-eukDB. The purpose of MT-eukDB was to use the protein sequences as a database to identify peptides derived from uncultured rumen fungi within a generated metaproteome data.源自真核生物、经聚腺苷酸（PolyA）富集提取的宏转录组（metatranscriptome，命名为‘MT-eukDB’），其原始测序数据来自瘤胃孵育过程中定殖于粉碎柳枝稷的真菌群落，共回收得到423,409,432条原始Illumina测序读段，总数据量约为63.5 Gbps。对原始读段完成质量过滤与序列组装后，我们共鉴定得到4,581,844个表达基因，其中4,550,231个（占比99.31%）被预测可编码蛋白质。在构建MT-eukDB之前，我们已将组装得到的宏转录组与截至2017年公开的可培养真菌基因组进行了比对过滤。MT-eukDB的构建目的是将其包含的蛋白质序列作为数据库，用于在已生成的宏蛋白质组（metaproteome）数据中鉴定源自未培养瘤胃真菌的肽段。