Warming effects on lizard gut microbiome depend on habitat connectivity

Climate warming and landscape fragmentation are both factors well known to threaten biodiversity and to generate species responses and adaptation. However, the impact of warming and fragmentation interplay on organismal responses remains largely under-explored, especially when it comes to gut symbionts, which may play a key role in essential host functions and traits by extending its functional and genetic repertoire. Here, we experimentally examined the combined effects of climate warming and habitat connectivity on the gut bacterial communities of the common lizard (Zootoca vivipara) over three years. While the strength of effects varied over the years, we found that a 2°C warmer climate decreases lizard gut microbiome diversity in isolated habitats. However, enabling connectivity among habitats with warmer and cooler climates offset or even reversed warming effects. The warming effects and the association between host dispersal behaviour and microbiome diversity appear to be a potent..., , ,

# File : MS1\_rawdata.tar.gz

Contains raw sequencing data (R1 and R2 fastq files). Number of raw sequencing reads in R1 and R2 files: 5899356 reads each.

# File : MS1\_ngsfilter\_16S\_Lez\_2015\_2018.tsv

Tab-sep text file containing the information to retrieve the sample origin of each sequencing reads. Provided in a format compatible with the use of OBITools.

# File : MS1\_obitab.tsv

Contains an MOTU (Molecular Operational Taxonomic Unit) by sample table after basic bioinformatic analysis with the OBITools package.\
Basic bioinformatics procedure includes reads pairing (default parameters), demultiplexing (2 errors on primers, 0 on tags), exclusion of sequences with counts = 1 (i.e. singletons), too short (>100 bp) or containing ambiguous bases, and clustering into OTUs at 97% sequence similarity

Number of sequencing reads : 11183625

Number of OTUs 97% : 8070

Description of the variables :

* GC_content: %GC of the MOTU sequence
* ID: Identification name of the sampl...

气候变暖与景观破碎化均为已知的威胁生物多样性、并可引发物种响应与适应性演化的关键因素。然而，二者的交互作用对生物个体响应的影响仍未得到充分探索，尤其是在肠道共生菌方面——这类微生物可通过拓展宿主的功能与遗传储备，在宿主核心生理功能与性状塑造中发挥关键作用。本研究历时三年，通过实验探究了气候变暖与栖息地连通性的交互作用对胎生蜥蜴（Zootoca vivipara）肠道细菌群落的影响。尽管效应强度随年份有所波动，但研究发现，在孤立栖息地中，2℃的气候变暖会降低蜥蜴肠道微生物组的多样性；而在存在冷暖气候差异的栖息地间建立连通性，则可抵消甚至逆转气候变暖的负面影响。气候变暖的效应以及宿主扩散行为与微生物组多样性之间的关联，似乎具备强大的……

# 文件：MS1_rawdata.tar.gz
包含原始测序数据（R1与R2双端fastq文件）。R1与R2文件中的原始测序读段数均为5899356条。

# 文件：MS1_ngsfilter_16S_Lez_2015_2018.tsv
制表符分隔文本文件，用于检索每条测序读段的样本来源，格式适配OBITools工具包的使用要求。

# 文件：MS1_obitab.tsv
包含经OBITools工具包完成基础生物信息学分析后得到的样本-分子操作分类单元（MOTU, Molecular Operational Taxonomic Unit）计数表。
基础生物信息学分析流程包括：读段配对（采用默认参数）、双端解复用（引物允许2个错配，标签允许0个错配）、剔除计数为1的序列（即单序列）、长度过短（<100 bp）或包含模糊碱基的序列，并以97%序列相似度聚类为OTU。
测序读段总数：11183625条
97%相似度OTU数量：8070个

变量说明：
* GC_content：MOTU序列的GC百分含量
* ID：样本的识别名称……