Differences in tissue populations following hematopoietic stem cell transplantation in Crohn’s disease patients. Differences in tissue populations following hematopoietic stem cell transplantation in Crohn’s disease patients

Treatment of severely refractory Crohn’s disease (CD) patients remains a clinical challenge. Recent studies show the efficacy of autologous hematopoietic stem cell transplant (HSCT) in these severely compromised patients. HSCT is thought to eliminate auto-reactive cells; however, no specific studies of immune reconstitution in CD patients are available. We studied a group of CD patients (n=18) receiving autologous HSCT and non-IBD patients (n=19) to elucidate the mechanism of interaction with the microorganisms. Overall design: Biopsy mRNA profiles of CD patients undergoing HSCT were generated by deep sequencing, using HiSeq4000 platform (Illumina, San Diego, CA)