Relative expression of the rhopalial opsins based on quantitative PCR.

Quantitative PCR ascertain the relative expression of Tc-leo and Tc-neo. In every run of the qPCR Tc-leo expressed relatively higher than Tc-neo. In every run the cycle threshold (CT) was lower for Tc-leo. In four runs Tc-neo was not sufficiently expressed to return a CT ascertaining the much higher relative expression of Tc-leo.本研究采用定量聚合酶链反应（Quantitative PCR，qPCR）检测Tc-leo与Tc-neo的相对表达水平。在每一轮qPCR实验中，Tc-leo的相对表达量均高于Tc-neo。每一轮实验中，Tc-leo的循环阈值（CT）均低于Tc-neo。在4轮实验中，Tc-neo的表达量不足以获得可检测的CT值，这进一步证实了Tc-leo相对表达量远高于Tc-neo。