Microsatellite genetic variation data for endangered Florida torreya (Torreya taxifolia Arn.)

This data publication includes allele calls for microsatellite markers for Torreya taxifolia Arn. (Florida torreya) a critically endangered (IUCN 3.1) conifer with a very limited native range in the USA, occurring in two counties in Florida and one in Georgia where it persists in ravines along the Apalachicola River. The objectives of this work are to develop genetic markers to uniquely identify individuals, examine the structure of the in situ population, and use an ex situ germplasm resource to determine if reproduction without fertilization (apomixis) occurs. We developed a panel of microsatellite markers, sampled individuals in 2008-2010 from three natural in situ populations in the Florida Gulf Coast and found population structure at all levels tested. The markers were next applied to ex situ mothers, potential fathers, and progeny to investigate the occurrence of apomixis. Data include both in situ and ex situ amplicon lengths for the 12 microsatellite loci (base pair).The objectives of this work are to develop genetic markers to uniquely identify individuals, examine the structure of the in situ population, and use an ex situ germplasm resource to determine if reproduction without fertilization (apomixis) occurs.For more information about these data and this study, see Dreaden et al. (2024).本数据集包含佛罗里达榧（Torreya taxifolia Arn.）的微卫星标记（microsatellite markers）等位基因分型结果。佛罗里达榧是一种极危（IUCN 3.1）针叶树种，原生分布范围极窄，仅见于美国佛罗里达州2个县与佐治亚州1个县，栖息于阿巴拉契科拉河沿岸的沟壑生境中。本研究的核心目标为：开发可唯一识别个体的遗传标记，解析原位种群（in situ population）的遗传结构，并借助迁地种质资源（ex situ germplasm resource），探究无需受精的生殖方式——无融合生殖（apomixis）是否存在。我们研发了一套微卫星标记组合，并于2008至2010年对佛罗里达墨西哥湾沿岸的3个天然原位种群开展个体采样，在所有检测水平下均检测到种群遗传结构。随后将该套标记应用于迁地母本、潜在父本及子代样本，以探究无融合生殖的发生情况。本数据集涵盖12个微卫星位点（microsatellite loci）的扩增片段长度（以碱基对（base pair，bp）为单位），包含原位种群与迁地种群两类样本的相关数据。本研究的目标为开发可唯一识别个体的遗传标记，解析原位种群的遗传结构，并利用迁地种质资源探究无受精生殖（即无融合生殖）是否发生。如需获取本数据集与本研究的更多详细信息，请参阅Dreaden等（2024）的研究文献。