Experimentally verified targets for miR-206.

*Hif1a upregulation in protein level, downregulation in mRNA level and LUC assay, **miR-206 can stimulate (normal cells) or repress (cancer cells) KLF4 translation. Abbreviations: LUC, luciferase assay; WB, western blot; qPCR, quantitative PCR; NB, northern blot; HEK293, human embryonic kidney cells; NIH10T1/2, fibroblasts; MCF-7, breast cancer cell line; MDA-MB-231, human adenocarcinoma cells; miR-206-KO,miR-206 knockout mouse; PASMC, pulmonary artery smooth muscle cells; HMEC, human mammary epithelial cells; RK3E, rat kidney cells; MCF10A, mammary epithelial cells; NIH3T3, mouse embryonic fibroblast cells; DF1, chicken embryonic fibroblasts; U343, human glioma cells; SK-N-SH, human neuroblastoma cells; C2C12, immortalized mouse skeletal myoblasts; SMSC, mouse skeletal muscle satellite cells; RuGli, rat glioma cells; COS7, African green monkey kidney cells; AML12, mouse hepatocytes; RMS, rhabdomyosarcoma cells; GC, human gastric cancer cells; HEK293T, human embryonic kidney cells with SV40 Large T-antigen; N2a, mouse neuroblastoma cells; SH-SY5Y, human neuroblastoma cells; MEF, mouse embryonic fibroblasts; MSC, human mesenchymal stem cells; MSC-NC, human mesenchymal stem cell-derived neural cells; HeLa, human epithelial cells from cervical carcinoma; CF, cardiac fibroblasts; CG, rat oligodendrocyte progenitor cells; H441, human lung adenocarcinoma epithelial cells.*Hif1a（缺氧诱导因子1α）在蛋白质水平上调、mRNA水平下调，且经荧光素酶检测（LUC，luciferase assay）验证，miR-206（微小RNA-206）可调控KLF4（Krüppel样因子4）的翻译：在正常细胞中发挥激活作用，在癌细胞中则发挥抑制作用。术语缩写说明：LUC（荧光素酶检测，luciferase assay）；WB（蛋白质免疫印迹，western blot）；qPCR（实时定量聚合酶链式反应，quantitative PCR）；NB（Northern印迹杂交，northern blot）；HEK293（人胚肾细胞，human embryonic kidney cells）；NIH10T1/2（成纤维细胞，fibroblasts）；MCF-7（乳腺癌细胞系，breast cancer cell line）；MDA-MB-231（人腺癌细胞，human adenocarcinoma cells）；miR-206-KO（miR-206敲除小鼠，miR-206 knockout mouse）；PASMC（肺动脉平滑肌细胞，pulmonary artery smooth muscle cells）；HMEC（人乳腺上皮细胞，human mammary epithelial cells）；RK3E（大鼠肾细胞，rat kidney cells）；MCF10A（乳腺上皮细胞，mammary epithelial cells）；NIH3T3（小鼠胚胎成纤维细胞，mouse embryonic fibroblast cells）；DF1（鸡胚成纤维细胞，chicken embryonic fibroblasts）；U343（人胶质瘤细胞，human glioma cells）；SK-N-SH（人神经母细胞瘤细胞，human neuroblastoma cells）；C2C12（永生化小鼠骨骼肌成肌细胞，immortalized mouse skeletal myoblasts）；SMSC（小鼠骨骼肌卫星细胞，mouse skeletal muscle satellite cells）；RuGli（大鼠胶质瘤细胞，rat glioma cells）；COS7（非洲绿猴肾细胞，African green monkey kidney cells）；AML12（小鼠肝细胞，mouse hepatocytes）；RMS（横纹肌肉瘤细胞，rhabdomyosarcoma cells）；GC（人胃癌细胞，human gastric cancer cells）；HEK293T（携带SV40大T抗原的人胚肾细胞，human embryonic kidney cells with SV40 Large T-antigen）；N2a（小鼠神经母细胞瘤细胞，mouse neuroblastoma cells）；SH-SY5Y（人神经母细胞瘤细胞，human neuroblastoma cells）；MEF（小鼠胚胎成纤维细胞，mouse embryonic fibroblasts）；MSC（人间充质干细胞，human mesenchymal stem cells）；MSC-NC（人源间充质干细胞来源神经细胞，human mesenchymal stem cell-derived neural cells）；HeLa（人宫颈癌细胞，human epithelial cells from cervical carcinoma）；CF（心脏成纤维细胞，cardiac fibroblasts）；CG（大鼠少突胶质前体细胞，rat oligodendrocyte progenitor cells）；H441（人肺腺癌上皮细胞，human lung adenocarcinoma epithelial cells）