The tricarboxylic acid cycle is inhibited under acute stress from carbonate alkalinity in the gills of <i>Eriocheir sinensis ——</i> Proteomics data

The TCA cycle in the gills is inhibited under acute carbonate alkalinity stress, according to proteomic and metabolomic analyses. The expressions of six enzymes, namely aconitate hydratase, isocitrate dehydrogenase, 2-oxoglutarate dehydrogenase, dihydrolipoyl dehydrogenase, succinate-CoA ligase, and malate dehydrogenase, were downregulated, resulting in the accumulation of phosphoenolpyruvic acid, citric acid, cis-aconitate, and a-ketoglutaric acid.通过蛋白质组学与代谢组学分析可知，急性碳酸盐碱胁迫下，鳃中的三羧酸循环（TCA cycle）受到抑制。六种酶即顺乌头酸水合酶、异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、二氢硫辛酰胺脱氢酶、琥珀酰-CoA合成酶以及苹果酸脱氢酶的表达量均出现下调，进而导致磷酸烯醇式丙酮酸、柠檬酸、顺乌头酸与α-酮戊二酸发生积累。