Additional file 1: of Increased expression of Cks1 protein is associated with lymph node metastasis and poor prognosis in nasopharyngeal carcinoma

The original data of IHC scoring. The expression of CKS1 and p27KIP1 had been tested in 168 cases of paraffin-embedded tissue from the primary NPC patients and 49 cases of non-cancerous nasopharyngeal control specimen from independent patients by IHC. The percentage of positive cells was divided into five grades (percentage scores):<10% (0), 11–25% (1), 26–50% (2), 51–75%(3), and >75% (4). The intensity of staining was divided into four grades (intensity scores): no staining (0), light brown (1), brown (2),and dark brown (3). Staining positivity was determined by the formula: overall scores = percentage score × intensity score. The total score ranged from 0 to 12, with negative staining (0–1) and positive expression (2–12). At last, 0 presented negative staining and 1 presented positive staining in the data file. (XLS 42 kb)

本数据集为免疫组化（Immunohistochemistry, IHC）评分原始数据集。研究采用免疫组化方法，对168例原发性鼻咽癌（Nasopharyngeal Carcinoma, NPC）患者的石蜡包埋组织样本，以及49例来自独立患者的非癌性鼻咽对照标本中的CKS1与p27KIP1表达水平进行了检测。阳性细胞占比被划分为五个等级（百分比评分）：<10%记0分，11%–25%记1分，26%–50%记2分，51%–75%记3分，>75%记4分。染色强度被划分为四个等级（强度评分）：无染色记0分，浅棕色记1分，棕色记2分，深棕色记3分。染色阳性情况通过以下公式判定：总评分=百分比评分×强度评分。总评分区间为0至12分，其中0–1分为阴性染色，2–12分为阳性表达。最终数据文件中以0代表阴性染色，1代表阳性表达。本数据集为大小42KB的XLS格式文件。