Summary of experimental validation results for the five designed sequences^a.

aThe sign of “+” and “−” indicates positive and negative experimental results respectively. bRMSD between the first I-TASSER model and the target scaffold. cProtein expression and solubility determined by visual identification via comassie stain gels. dPresence of secondary structural elements defined by circular dichroism. ePossession of a stable tertiary fold determined by the presence of secondary structural elements (CD) and NMR spectroscopy. fPercentage of α-helix residues decided by the CD spectra (the values in parentheses are the number in the scaffold structure.