Single-Cell Sequencing Reveals the Impact of Mild Traumatic Brain Injury on the Hippocampus

To elucidate molecular features of the cells of Hippocampus, their progeny at each differentiation stage, and surrounding cells in hippocampus undercontrolled cortical impact (CCI) conditions, >15000 cells were profiled from matched hippocampus of 3 mice that had received CCI and 3 sham-operated controls.Cell type specific gene expression responding to (mild traumatic brain injury, mTBI) were revealed, some of which were closely associated with pathogenesis post-injury. Furthermore, the diverse cell to cell interaction networks uncovered an array of cellular processes under mTBI. Our gene expression atlas provides extensive resources for future researches regarding mTBI induced pathogenesis, its therapeutic interventions or diagnostic tests. Overall design: Hippocampus mRNA profiles of 8~10-week old mTBI and Sham mice were generated by scRNA-Seq.