AlphaFold 3 predicted structure and PyMOL-based interaction analysis of [Cx43] and [Smurf2]

Protein structure prediction was performed using AlphaFold 3. The top-ranked model (ranked_0), as determined by the internal ranking confidence metrics, was selected for downstream analysis. Structural visualization and interaction analysis were conducted using PyMOL. The ranked_0 model was used to identify putative binding interfaces and key interacting residues.本研究采用AlphaFold 3完成蛋白质结构预测。依据内部排名置信度指标筛选出排名首位的模型（ranked_0），用于下游分析。借助PyMOL开展结构可视化与相互作用分析。利用该ranked_0模型，可识别推定的结合界面与关键相互作用残基。