Oil solubilization and degradation by exopolysaccharide producing Halomonas strains

Cultured isolates of the genus Halomonas were tested for the ability to emulsify and degrade aromatic hydrocarbons as well as their ability to form oil aggregates. High-throughput DNA sequencing was also used to determine the ambient abundance of these culture strains during the Deepwater Horizon blowout. Pyrosequencing data were submitted to the European Nucleotide Archive Sequence Read Archive under the study accession number ERP002443. The 16S rRNA gene sequence of Halomonas strains GOS-2, GOS-3a and TGOS-10 were deposited with GenBank under accession numbers JQ246430, JQ246431 and JQ246432, respectively. This dataset reports disintegrations per minutes (dmp), hydrocarbon concentrations, and absorbance from experiments reported in the publication Gutirrez et al 2013, Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill, PLOS ONE, 8 (6):e67717 doi:10.1371/journal.pone.0067717.

本研究针对盐单胞菌属（Halomonas）的培养分离株，检测其乳化、降解芳香烃（aromatic hydrocarbons）的能力，以及形成油聚集体（oil aggregates）的能力。同时借助高通量DNA测序（high-throughput DNA sequencing）技术，测定了深水地平线井喷（Deepwater Horizon blowout）事件期间这些培养菌株的环境丰度。本研究将焦磷酸测序（Pyrosequencing）数据提交至欧洲核苷酸档案馆序列读取存档库（European Nucleotide Archive Sequence Read Archive），研究收录编号为ERP002443。盐单胞菌菌株GOS-2、GOS-3a及TGOS-10的16S rRNA基因序列已分别提交至GenBank数据库，收录编号依次为JQ246430、JQ246431与JQ246432。本数据集收录了Gutirrez等人2013年发表于《公共科学图书馆·综合》（PLOS ONE）的实验数据，具体文献信息为：《细菌胞外多糖（Exopolysaccharides, EPS）在深水地平线漏油事件中释放石油的归趋中的作用（Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill）》，刊载于2013年，第8卷第6期，文章编号为e67717，DOI:10.1371/journal.pone.0067717，数据内容包括每分钟衰变次数（disintegrations per minutes, dpm）、烃类浓度与吸光度值。