EBNA-1 quasispecies detected with FLX sequencing.

Minority EBV sequence variants at a level of ≥10% were detected in two samples only. Sequence mixtures present at a ≥5% threshold revealed minority variants in 3.6% of investigated nucleotide positions (27/749 nucleotides sequenced,), increasing to 5.0% at a ≥2% cut-off (37/749 nucleotides,) and 8.3% at a ≥1% cut-off (62/749 nucleotides,). Samples 7, 8 and 13 did not have minority species present at ≥1%.仅在2份样本中检测到丰度≥10%的EB病毒（Epstein-Barr Virus, EBV）序列低频变异体。当以≥5%为阈值判定存在序列混合现象时，在所检测的749个核苷酸位点中，3.6%的位点（27/749个测序核苷酸）存在低频变异体；将截值设为≥2%时，该比例升至5.0%（37/749个测序核苷酸）；截值设为≥1%时，比例进一步提升至8.3%（62/749个测序核苷酸）。样本7、8及13未检出丰度≥1%的低频变异体。