Supporting data for “Construction of Photo-responsive Nanomaterials and Their Application in Multidrug-resistant Bacteria-infected Wounds Therapy”

In this study, a novel photo-responsive nanomaterial has been developed. The material morphology was measured by TEM and SEM,  and the physicochemical properties have described by the XPS, FT-IR, and XRD spectra. In addition, the DFT calculation was used for revealing the charge transfer route and mechanism of ROS generation. The results of electron paramagnetic resonance indicate that the photo-responsive nanomaterial can generate different levels of ROS by light regulation. And the photo-responsive nanomaterial realized the rapid sterilization and promoting angiogenesis in vitro and in vivo. The animal study indicated that the photo-responsive nanomaterial has a positive effect on bacteria-infected wound healing. The developed strategy to integrate the photo-responsive nanomaterial and biological effects of ROS could benefit the development of clinical treatments for bacteria-infected tissues.

在本项研究中，一种新型的光响应性纳米材料得以开发。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）对材料形貌进行了测量，并利用X射线光电子能谱（XPS）、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）谱对其物理化学性质进行了描述。此外，密度泛函理论（DFT）计算被用于揭示活性氧（ROS）生成过程中的电荷转移路径和机制。电子顺磁共振的结果表明，该光响应性纳米材料能够通过光调节生成不同级别的ROS。该光响应性纳米材料在体外和体内实现了快速灭菌和促进血管生成。动物实验表明，光响应性纳米材料对细菌感染伤口愈合具有积极影响。将光响应性纳米材料与ROS的生物效应相结合的开发策略，有望促进针对细菌感染组织的临床治疗方法的研发。