Single-cell RNA sequencing of immune cells from control or PHGDH knockdown liver tumours under irradiation treatment.

To enhance the efficacy of radio-immunotherapy and sensitize tumors to treatment, we employed an in vivo screening method to identify key metabolic enzymes involved in therapy response. We discovered that PHGDH, a limited metabolic enzyme involved in de novo serine synthesis, targets the LATS1/2 kinase and activates the YAP1 nuclear translocation and its noncanonical signal transduction under irradiation treatment, thereby promoting tumor tolerance.The goal of the study is to profile the change of immune cells by the knockdown of PHGDH in liver tumours formed by Hepa1-6 cells.