ABO methylation analysis in patient specimens with ABO allelic loss of expression.

In the ID column a F prefix denotes patients analyzed by flow cytometry while a S prefix denotes loss of ABH antigen patients as detected by serology. ‘GENO’ refers to ABO genotype, ‘METH’ to ABO promoter CpG island methylation assessed either by MS-SSCA, COBRA and/or melt curve analysis (MCA). ‘U’ unmethylated at the ABO promoter and ‘M’ is methylated at the ABO promoter. For patient F60 two samples were available for analysis; the 1993 ('93) sample was positive for ABO expression whereas the 1996 ('96) sample was negative for ABO expression.在ID列中，前缀F代表经流式细胞术（flow cytometry）分析的患者，前缀S则代表经血清学（serology）检测确认ABH抗原缺失的患者。"GENO"指代ABO基因型（ABO genotype），"METH"指代通过MS-SSCA、COBRA及（或）熔解曲线分析（melt curve analysis, MCA）评估的ABO启动子CpG岛甲基化状态。其中"U"表示ABO启动子未发生甲基化，"M"表示ABO启动子发生甲基化。患者F60拥有两份可供分析的样本：1993年（'93）的样本ABO表达呈阳性，而1996年（'96）的样本ABO表达呈阴性。