Interleukin-6 from the Adipose Secretome Potentiate Differentiation of Adipose Progenitors Through Activation of Redox Signaling

Supplementary Figure 1. Adipogenic Stimulus Increases the Number of Short Mitochondria. (A) Box plot of mitochondrial length analyzed by category, short, intermediate, or elongated for APCs treated adipogenic stimulus or vehicle normalized to cell number per slide in mitotracker-stained APCs (B) Representative images of mitotracker stained APCs.
Supplementary Figure 2. Conditioned Media Treatment Increases Adipogenesis. (A) Eluted Oil Red O absorbance for cells treated with CD CM or HFD CM from male subcutaneous adipose tissue explants or vehicle on day 7 of differentiation (n=4). * p ≤ 0.05.
Supplementary Figure 3. IL-6 and TNF Identified as Upstream Regulators (A) Network of IL-6 as an upstream regulator of differentially expressed proteins found in our dataset in IPA. (B) Network of TNFa as an upstream regulator of differentially expressed proteins found in our dataset in IPA.
Supplementary Figure 4. IL-6 Treatment Increases Adipogenesis. (A) Eluted Oil Red O absorbance from APCs treated with IL-6 [0.1, 1, 5 ng/mL] (n=4). * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.